Computational Design of Nucleic Acid Circuits: Past, Present, and Future

Abstract Over the past 40 years, significant progress has been made on design and implementation of nucleic acid circuits, which represent computational core dynamic DNA nanotechnology. This enabled primarily by substantial advances in experimental techniques, but also parallel methods for circuit design. In this perspective, we look back at evolution these through lens Visual DSD system, developed over decade analysis circuits. We trace time relation to more broadly, outline how have tried keep pace with rapid techniques. Along way, summarize key theoretical concepts from computer science mathematics that underpin methods, weaving them together using a common running example simple Join circuit. On occasion 40th anniversary nanotechnology, offer some thoughts possible future directions circuits may influence, be influenced by, developments.